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Human Protein Atlas immunohistochemistry ihc staining 764 images
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Related Articles

Immunohistochemistry:

Article Title: Spatially resolved transcriptomic analysis of the adult human prostate.
Article Snippet: The prostate is an organ characterized by significant spatial heterogeneity.. To better understand its intricate structure and cellular composition, we constructed a comprehensive single-cell atlas of the adult human prostate.. Our high-resolution mapping effort identified 253,381 single cells and 34,876 nuclei sampled from 11 patients who underwent radical resection of bladder cancer, which were categorized into 126 unique subpopulations.

Article Title: From foe to friend: Rewiring oncogenic pathways through artificial selenoprotein to combat immune-resistant tumor
Article Snippet: .. Furthermore, consistent with these results, IHC staining images from the human protein atlas (HPA) database demonstrated elevated levels of MDM2 protein in colon cancer tissues when compared to normal tissues ( ). ..

Article Title: Helicobacter pylori promotes gastric cancer progression via UPP1-mediated uridine bypass of glycolysis
Article Snippet: .. Additionally, protein expression data were retrieved from the Human Protein Atlas (HPA), including IHC staining images, providing further insights into UPP1 expression at the protein level. ..

Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
Article Snippet: Although NanoString analyses revealed the presence of arginase−1 transcripts ( ARG1 ) in bulk melanoma biopsies , in vitro experiments with eight different melanoma cell lines showed no detectable release of arginase−1, even after stimulation with TNF, a known inducer of arginase−1 exocytosis ( Figure S7A ). .. Analysis of IHC staining images from the Human Protein Atlas (proteinatlas.org) showed that arginase−1 was largely undetectable in the cutaneous melanoma TME ( Figure S7B ). ..

Article Title: Characterization of the Biochemical Recurrence Prediction Ability and Progression Correlation of Peroxiredoxins Family in Prostate Cancer Based on Integrating Single‐Cell RNA ‐Seq and Bulk RNA ‐Seq Cohorts
Article Snippet: .. We accessed IHC staining images of PRDX5 in normal prostate tissue, low‐grade, and high‐grade prostate cancer tissue from the Human Protein Atlas database to preliminarily identify the expression pattern of PRDX5. .. Subsequently, we employed IHC to detect the expression of PRDX5 (67599‐1‐Ig, Proteintech) in Chinese tissue microarrays (HProA150CS01, OUTDO BIOTECH), employing adjacent non‐tumor prostate tissue and tumor tissues of different pathological T‐stages of prostate cancer.

Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
Article Snippet: .. Although NanoString analyses revealed the presence of arginase−1 transcripts (ARG1) in bulk melanoma biopsies (Figure 4A), in vitro experiments with eight different melanoma cell lines showed no detectable release of arginase−1, even after stimulation with TNF, a known inducer of arginase−1 exocytosis (Figure S7A).9 Analysis of IHC staining images from the Human Protein Atlas (proteinatlas.org) showed that arginase−1 was largely undetectable in the cutaneous melanoma TME (Figure S7B).21 Among the 26 biopsies analyzed, only a subset exhibited low-intensity staining, with cytosolic/membrane localization in four cases and nuclear staining in another four, suggesting its limited expression in melanoma.21 In peripheral blood, NanoString analyses detected ARG1 expression in monocytes, but not in lymphocytes (Figure 4A), a trend consistent throughout the trial period. ..

Article Title: Helicobacter pylori promotes gastric cancer progression via UPP1-mediated uridine bypass of glycolysis.
Article Snippet: .. Additionally, protein expression data were retrieved from the Human Protein Atlas (HPA), including IHC staining images, providing further insights into UPP1 expression at the protein level. ..

Article Title: Identification of SAMD9 as an adaptive response gene to environmental changes and its association with overall survival and immunotherapeutic response in glioblastoma.
Article Snippet: .. 6 IHC staining images from the Human Protein Atlas and and proteomic data from 7 the UALCAN portal confirmed SAMD9 protein overexpression in glioblastoma 8 relative to normal brain and low-grade gliomas (Fig. S2E-F). ..

Staining:

Article Title: Spatially resolved transcriptomic analysis of the adult human prostate.
Article Snippet: The prostate is an organ characterized by significant spatial heterogeneity.. To better understand its intricate structure and cellular composition, we constructed a comprehensive single-cell atlas of the adult human prostate.. Our high-resolution mapping effort identified 253,381 single cells and 34,876 nuclei sampled from 11 patients who underwent radical resection of bladder cancer, which were categorized into 126 unique subpopulations.

Article Title: From foe to friend: Rewiring oncogenic pathways through artificial selenoprotein to combat immune-resistant tumor
Article Snippet: .. Furthermore, consistent with these results, IHC staining images from the human protein atlas (HPA) database demonstrated elevated levels of MDM2 protein in colon cancer tissues when compared to normal tissues ( ). ..

Article Title: Helicobacter pylori promotes gastric cancer progression via UPP1-mediated uridine bypass of glycolysis
Article Snippet: .. Additionally, protein expression data were retrieved from the Human Protein Atlas (HPA), including IHC staining images, providing further insights into UPP1 expression at the protein level. ..

Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
Article Snippet: Although NanoString analyses revealed the presence of arginase−1 transcripts ( ARG1 ) in bulk melanoma biopsies , in vitro experiments with eight different melanoma cell lines showed no detectable release of arginase−1, even after stimulation with TNF, a known inducer of arginase−1 exocytosis ( Figure S7A ). .. Analysis of IHC staining images from the Human Protein Atlas (proteinatlas.org) showed that arginase−1 was largely undetectable in the cutaneous melanoma TME ( Figure S7B ). ..

Article Title: Characterization of the Biochemical Recurrence Prediction Ability and Progression Correlation of Peroxiredoxins Family in Prostate Cancer Based on Integrating Single‐Cell RNA ‐Seq and Bulk RNA ‐Seq Cohorts
Article Snippet: .. We accessed IHC staining images of PRDX5 in normal prostate tissue, low‐grade, and high‐grade prostate cancer tissue from the Human Protein Atlas database to preliminarily identify the expression pattern of PRDX5. .. Subsequently, we employed IHC to detect the expression of PRDX5 (67599‐1‐Ig, Proteintech) in Chinese tissue microarrays (HProA150CS01, OUTDO BIOTECH), employing adjacent non‐tumor prostate tissue and tumor tissues of different pathological T‐stages of prostate cancer.

Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
Article Snippet: .. Although NanoString analyses revealed the presence of arginase−1 transcripts (ARG1) in bulk melanoma biopsies (Figure 4A), in vitro experiments with eight different melanoma cell lines showed no detectable release of arginase−1, even after stimulation with TNF, a known inducer of arginase−1 exocytosis (Figure S7A).9 Analysis of IHC staining images from the Human Protein Atlas (proteinatlas.org) showed that arginase−1 was largely undetectable in the cutaneous melanoma TME (Figure S7B).21 Among the 26 biopsies analyzed, only a subset exhibited low-intensity staining, with cytosolic/membrane localization in four cases and nuclear staining in another four, suggesting its limited expression in melanoma.21 In peripheral blood, NanoString analyses detected ARG1 expression in monocytes, but not in lymphocytes (Figure 4A), a trend consistent throughout the trial period. ..

Article Title: Helicobacter pylori promotes gastric cancer progression via UPP1-mediated uridine bypass of glycolysis.
Article Snippet: .. Additionally, protein expression data were retrieved from the Human Protein Atlas (HPA), including IHC staining images, providing further insights into UPP1 expression at the protein level. ..

Article Title: Identification of SAMD9 as an adaptive response gene to environmental changes and its association with overall survival and immunotherapeutic response in glioblastoma.
Article Snippet: .. 6 IHC staining images from the Human Protein Atlas and and proteomic data from 7 the UALCAN portal confirmed SAMD9 protein overexpression in glioblastoma 8 relative to normal brain and low-grade gliomas (Fig. S2E-F). ..

RNA Sequencing:

Article Title: Spatially resolved transcriptomic analysis of the adult human prostate.
Article Snippet: The prostate is an organ characterized by significant spatial heterogeneity.. To better understand its intricate structure and cellular composition, we constructed a comprehensive single-cell atlas of the adult human prostate.. Our high-resolution mapping effort identified 253,381 single cells and 34,876 nuclei sampled from 11 patients who underwent radical resection of bladder cancer, which were categorized into 126 unique subpopulations.

Expressing:

Article Title: Helicobacter pylori promotes gastric cancer progression via UPP1-mediated uridine bypass of glycolysis
Article Snippet: .. Additionally, protein expression data were retrieved from the Human Protein Atlas (HPA), including IHC staining images, providing further insights into UPP1 expression at the protein level. ..

Article Title: Characterization of the Biochemical Recurrence Prediction Ability and Progression Correlation of Peroxiredoxins Family in Prostate Cancer Based on Integrating Single‐Cell RNA ‐Seq and Bulk RNA ‐Seq Cohorts
Article Snippet: .. We accessed IHC staining images of PRDX5 in normal prostate tissue, low‐grade, and high‐grade prostate cancer tissue from the Human Protein Atlas database to preliminarily identify the expression pattern of PRDX5. .. Subsequently, we employed IHC to detect the expression of PRDX5 (67599‐1‐Ig, Proteintech) in Chinese tissue microarrays (HProA150CS01, OUTDO BIOTECH), employing adjacent non‐tumor prostate tissue and tumor tissues of different pathological T‐stages of prostate cancer.

Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
Article Snippet: .. Although NanoString analyses revealed the presence of arginase−1 transcripts (ARG1) in bulk melanoma biopsies (Figure 4A), in vitro experiments with eight different melanoma cell lines showed no detectable release of arginase−1, even after stimulation with TNF, a known inducer of arginase−1 exocytosis (Figure S7A).9 Analysis of IHC staining images from the Human Protein Atlas (proteinatlas.org) showed that arginase−1 was largely undetectable in the cutaneous melanoma TME (Figure S7B).21 Among the 26 biopsies analyzed, only a subset exhibited low-intensity staining, with cytosolic/membrane localization in four cases and nuclear staining in another four, suggesting its limited expression in melanoma.21 In peripheral blood, NanoString analyses detected ARG1 expression in monocytes, but not in lymphocytes (Figure 4A), a trend consistent throughout the trial period. ..

Article Title: Helicobacter pylori promotes gastric cancer progression via UPP1-mediated uridine bypass of glycolysis.
Article Snippet: .. Additionally, protein expression data were retrieved from the Human Protein Atlas (HPA), including IHC staining images, providing further insights into UPP1 expression at the protein level. ..

In Vitro:

Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
Article Snippet: .. Although NanoString analyses revealed the presence of arginase−1 transcripts (ARG1) in bulk melanoma biopsies (Figure 4A), in vitro experiments with eight different melanoma cell lines showed no detectable release of arginase−1, even after stimulation with TNF, a known inducer of arginase−1 exocytosis (Figure S7A).9 Analysis of IHC staining images from the Human Protein Atlas (proteinatlas.org) showed that arginase−1 was largely undetectable in the cutaneous melanoma TME (Figure S7B).21 Among the 26 biopsies analyzed, only a subset exhibited low-intensity staining, with cytosolic/membrane localization in four cases and nuclear staining in another four, suggesting its limited expression in melanoma.21 In peripheral blood, NanoString analyses detected ARG1 expression in monocytes, but not in lymphocytes (Figure 4A), a trend consistent throughout the trial period. ..

Over Expression:

Article Title: Identification of SAMD9 as an adaptive response gene to environmental changes and its association with overall survival and immunotherapeutic response in glioblastoma.
Article Snippet: .. 6 IHC staining images from the Human Protein Atlas and and proteomic data from 7 the UALCAN portal confirmed SAMD9 protein overexpression in glioblastoma 8 relative to normal brain and low-grade gliomas (Fig. S2E-F). ..



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Human Protein Atlas immunohistochemistry ihc staining 764 images
Immunohistochemistry Ihc Staining 764 Images, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas immunohistochemical staining images
Age‐dependent NTS molecule expression patterns across cell types. (A) Relative expression of NTS across cell types (ExN, InN, MOL, OPC, Astro, Micro, Endo, Peri) as a function of age. NTS include acetylcholine, cannabinoid, dopamine, GABA, glutamate, glycine, histamine, MAT (monoamine transporters), norepinephrine, opioid, progestin, prostaglandin, and serotonin. Notable trends include age‐associated increases or decreases in expression levels of specific neurotransmitters within distinct cell types. (B) <t>Immunohistochemical</t> staining of HTR1E in cerebral cortex tissue (HPA004931). Images show representative staining in male (age 45) and female (age 54) samples. Scale bar: 100 μm. (C) Scatter plot of normalized TPM (nTPM) values showing HTR1E expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: HTR1E high , HTR1E middle , and HTR1E low . (D) Bar plot showing the distribution of HTR1E expression clusters across young and old groups. The proportion of samples in each cluster is displayed. (E) Immunohistochemical staining of PGRMC1 in cerebral cortex tissue (HPA002877). Images show representative staining in female (age 45) and female (age 54) samples. Insets highlight specific regions with detailed staining patterns. Scale bar: 100 μm. (F) Scatter plot of normalized TPM (nTPM) values showing PGRMC1 expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: PGRMC1 high , PGRMC1 middle , and PGRMC1 low . (G) Bar plot showing the distribution of PGRMC1 expression clusters across young and old groups. The proportion of samples in each cluster is displayed.
Immunohistochemical Staining Images, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas immunohistochemical ihc staining images
Age‐dependent NTS molecule expression patterns across cell types. (A) Relative expression of NTS across cell types (ExN, InN, MOL, OPC, Astro, Micro, Endo, Peri) as a function of age. NTS include acetylcholine, cannabinoid, dopamine, GABA, glutamate, glycine, histamine, MAT (monoamine transporters), norepinephrine, opioid, progestin, prostaglandin, and serotonin. Notable trends include age‐associated increases or decreases in expression levels of specific neurotransmitters within distinct cell types. (B) <t>Immunohistochemical</t> staining of HTR1E in cerebral cortex tissue (HPA004931). Images show representative staining in male (age 45) and female (age 54) samples. Scale bar: 100 μm. (C) Scatter plot of normalized TPM (nTPM) values showing HTR1E expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: HTR1E high , HTR1E middle , and HTR1E low . (D) Bar plot showing the distribution of HTR1E expression clusters across young and old groups. The proportion of samples in each cluster is displayed. (E) Immunohistochemical staining of PGRMC1 in cerebral cortex tissue (HPA002877). Images show representative staining in female (age 45) and female (age 54) samples. Insets highlight specific regions with detailed staining patterns. Scale bar: 100 μm. (F) Scatter plot of normalized TPM (nTPM) values showing PGRMC1 expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: PGRMC1 high , PGRMC1 middle , and PGRMC1 low . (G) Bar plot showing the distribution of PGRMC1 expression clusters across young and old groups. The proportion of samples in each cluster is displayed.
Immunohistochemical Ihc Staining Images, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas immunohistochemistry ihc staining images
Age‐dependent NTS molecule expression patterns across cell types. (A) Relative expression of NTS across cell types (ExN, InN, MOL, OPC, Astro, Micro, Endo, Peri) as a function of age. NTS include acetylcholine, cannabinoid, dopamine, GABA, glutamate, glycine, histamine, MAT (monoamine transporters), norepinephrine, opioid, progestin, prostaglandin, and serotonin. Notable trends include age‐associated increases or decreases in expression levels of specific neurotransmitters within distinct cell types. (B) <t>Immunohistochemical</t> staining of HTR1E in cerebral cortex tissue (HPA004931). Images show representative staining in male (age 45) and female (age 54) samples. Scale bar: 100 μm. (C) Scatter plot of normalized TPM (nTPM) values showing HTR1E expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: HTR1E high , HTR1E middle , and HTR1E low . (D) Bar plot showing the distribution of HTR1E expression clusters across young and old groups. The proportion of samples in each cluster is displayed. (E) Immunohistochemical staining of PGRMC1 in cerebral cortex tissue (HPA002877). Images show representative staining in female (age 45) and female (age 54) samples. Insets highlight specific regions with detailed staining patterns. Scale bar: 100 μm. (F) Scatter plot of normalized TPM (nTPM) values showing PGRMC1 expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: PGRMC1 high , PGRMC1 middle , and PGRMC1 low . (G) Bar plot showing the distribution of PGRMC1 expression clusters across young and old groups. The proportion of samples in each cluster is displayed.
Immunohistochemistry Ihc Staining Images, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas representative 518 immunohistochemical staining images
Age‐dependent NTS molecule expression patterns across cell types. (A) Relative expression of NTS across cell types (ExN, InN, MOL, OPC, Astro, Micro, Endo, Peri) as a function of age. NTS include acetylcholine, cannabinoid, dopamine, GABA, glutamate, glycine, histamine, MAT (monoamine transporters), norepinephrine, opioid, progestin, prostaglandin, and serotonin. Notable trends include age‐associated increases or decreases in expression levels of specific neurotransmitters within distinct cell types. (B) <t>Immunohistochemical</t> staining of HTR1E in cerebral cortex tissue (HPA004931). Images show representative staining in male (age 45) and female (age 54) samples. Scale bar: 100 μm. (C) Scatter plot of normalized TPM (nTPM) values showing HTR1E expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: HTR1E high , HTR1E middle , and HTR1E low . (D) Bar plot showing the distribution of HTR1E expression clusters across young and old groups. The proportion of samples in each cluster is displayed. (E) Immunohistochemical staining of PGRMC1 in cerebral cortex tissue (HPA002877). Images show representative staining in female (age 45) and female (age 54) samples. Insets highlight specific regions with detailed staining patterns. Scale bar: 100 μm. (F) Scatter plot of normalized TPM (nTPM) values showing PGRMC1 expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: PGRMC1 high , PGRMC1 middle , and PGRMC1 low . (G) Bar plot showing the distribution of PGRMC1 expression clusters across young and old groups. The proportion of samples in each cluster is displayed.
Representative 518 Immunohistochemical Staining Images, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Human Protein Atlas representative immunohistochemical staining images
TMEM132A is upregulated in breast cancer and correlates with clinicopathological features ( A ) Differential expression of TMEM132A between breast cancer tissues and normal breast tissues in the TCGA_BRCA cohort. ( B ) TMEM132A expression comparison between tumor samples from TCGA_BRCA and normal breast tissues from the integrated TCGA and GTEx cohort. ( C-E ) Validation of elevated TMEM132A expression in breast cancer using independent GEO datasets, including GSE20711 ( C ), GSE22820 ( D ), and GSE162228 ( E ). ( F , G ) ROC curve analyses evaluating the diagnostic performance of TMEM132A in distinguishing breast cancer tissues from normal tissues in the TCGA_BRCA cohort ( F ) and the TCGA+GTEx_BRCA cohort ( G ). ( H ) Paired analysis of TMEM132A expression between matched tumor and adjacent normal breast tissues in the TCGA_BRCA cohort. ( I , J ) Association between TMEM132A expression and clinical stage in the TCGA_BRCA cohort. ( K ) Distribution of TMEM132A expression across different molecular subtypes of breast cancer in the TCGA_BRCA cohort. ( L-T ) Relationship between TMEM132A expression and histological grade in the TCGA_BRCA cohort (L) and multiple independent GEO datasets, including GSE7390 ( M ), GSE11121 ( N ), GSE20711 ( O ), GSE21653 ( P ), GSE22219 ( Q ), GSE25055 ( R ), GSE45255 ( S ), GSE42568 ( T ), and GSE61304 ( U ). ( U , V ) Comparison of TMEM132A expression between non-metastatic (M0) and metastatic (M1) breast cancer samples in the TCGA_BRCA cohort ( U ) and the GSE20685 dataset ( V ). ( W ) Distribution of TMEM132A expression levels across breast cancer molecular. subgroups in the TCGA_BRCA cohort. ( X ) Representative <t>immunohistochemical</t> staining images showing TMEM132A protein expression in normal breast tissue and breast cancer tissue obtained from the Human Protein Atlas. ( Y ) Immunofluorescence staining showing TMEM132A expression (green), nuclei (blue), microtubules (red), and merged images in the MCF7 breast cancer cell line
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Image Search Results


Age‐dependent NTS molecule expression patterns across cell types. (A) Relative expression of NTS across cell types (ExN, InN, MOL, OPC, Astro, Micro, Endo, Peri) as a function of age. NTS include acetylcholine, cannabinoid, dopamine, GABA, glutamate, glycine, histamine, MAT (monoamine transporters), norepinephrine, opioid, progestin, prostaglandin, and serotonin. Notable trends include age‐associated increases or decreases in expression levels of specific neurotransmitters within distinct cell types. (B) Immunohistochemical staining of HTR1E in cerebral cortex tissue (HPA004931). Images show representative staining in male (age 45) and female (age 54) samples. Scale bar: 100 μm. (C) Scatter plot of normalized TPM (nTPM) values showing HTR1E expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: HTR1E high , HTR1E middle , and HTR1E low . (D) Bar plot showing the distribution of HTR1E expression clusters across young and old groups. The proportion of samples in each cluster is displayed. (E) Immunohistochemical staining of PGRMC1 in cerebral cortex tissue (HPA002877). Images show representative staining in female (age 45) and female (age 54) samples. Insets highlight specific regions with detailed staining patterns. Scale bar: 100 μm. (F) Scatter plot of normalized TPM (nTPM) values showing PGRMC1 expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: PGRMC1 high , PGRMC1 middle , and PGRMC1 low . (G) Bar plot showing the distribution of PGRMC1 expression clusters across young and old groups. The proportion of samples in each cluster is displayed.

Journal: Aging Cell

Article Title: Multi‐Omics Reveals Dysregulated Neurotransmitter Systems in Aging and CNS Disorders

doi: 10.1111/acel.70544

Figure Lengend Snippet: Age‐dependent NTS molecule expression patterns across cell types. (A) Relative expression of NTS across cell types (ExN, InN, MOL, OPC, Astro, Micro, Endo, Peri) as a function of age. NTS include acetylcholine, cannabinoid, dopamine, GABA, glutamate, glycine, histamine, MAT (monoamine transporters), norepinephrine, opioid, progestin, prostaglandin, and serotonin. Notable trends include age‐associated increases or decreases in expression levels of specific neurotransmitters within distinct cell types. (B) Immunohistochemical staining of HTR1E in cerebral cortex tissue (HPA004931). Images show representative staining in male (age 45) and female (age 54) samples. Scale bar: 100 μm. (C) Scatter plot of normalized TPM (nTPM) values showing HTR1E expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: HTR1E high , HTR1E middle , and HTR1E low . (D) Bar plot showing the distribution of HTR1E expression clusters across young and old groups. The proportion of samples in each cluster is displayed. (E) Immunohistochemical staining of PGRMC1 in cerebral cortex tissue (HPA002877). Images show representative staining in female (age 45) and female (age 54) samples. Insets highlight specific regions with detailed staining patterns. Scale bar: 100 μm. (F) Scatter plot of normalized TPM (nTPM) values showing PGRMC1 expression levels in frontal cortex samples (BA9, n = 209). Samples are stratified into three clusters: PGRMC1 high , PGRMC1 middle , and PGRMC1 low . (G) Bar plot showing the distribution of PGRMC1 expression clusters across young and old groups. The proportion of samples in each cluster is displayed.

Article Snippet: Immunohistochemical staining images and frontal cortex RNA‐seq data from the Human Protein Atlas (HPA) provided supportive cross‐dataset validation for the snRNA‐seq findings.

Techniques: Expressing, Immunohistochemical staining, Staining

TMEM132A is upregulated in breast cancer and correlates with clinicopathological features ( A ) Differential expression of TMEM132A between breast cancer tissues and normal breast tissues in the TCGA_BRCA cohort. ( B ) TMEM132A expression comparison between tumor samples from TCGA_BRCA and normal breast tissues from the integrated TCGA and GTEx cohort. ( C-E ) Validation of elevated TMEM132A expression in breast cancer using independent GEO datasets, including GSE20711 ( C ), GSE22820 ( D ), and GSE162228 ( E ). ( F , G ) ROC curve analyses evaluating the diagnostic performance of TMEM132A in distinguishing breast cancer tissues from normal tissues in the TCGA_BRCA cohort ( F ) and the TCGA+GTEx_BRCA cohort ( G ). ( H ) Paired analysis of TMEM132A expression between matched tumor and adjacent normal breast tissues in the TCGA_BRCA cohort. ( I , J ) Association between TMEM132A expression and clinical stage in the TCGA_BRCA cohort. ( K ) Distribution of TMEM132A expression across different molecular subtypes of breast cancer in the TCGA_BRCA cohort. ( L-T ) Relationship between TMEM132A expression and histological grade in the TCGA_BRCA cohort (L) and multiple independent GEO datasets, including GSE7390 ( M ), GSE11121 ( N ), GSE20711 ( O ), GSE21653 ( P ), GSE22219 ( Q ), GSE25055 ( R ), GSE45255 ( S ), GSE42568 ( T ), and GSE61304 ( U ). ( U , V ) Comparison of TMEM132A expression between non-metastatic (M0) and metastatic (M1) breast cancer samples in the TCGA_BRCA cohort ( U ) and the GSE20685 dataset ( V ). ( W ) Distribution of TMEM132A expression levels across breast cancer molecular. subgroups in the TCGA_BRCA cohort. ( X ) Representative immunohistochemical staining images showing TMEM132A protein expression in normal breast tissue and breast cancer tissue obtained from the Human Protein Atlas. ( Y ) Immunofluorescence staining showing TMEM132A expression (green), nuclei (blue), microtubules (red), and merged images in the MCF7 breast cancer cell line

Journal: Clinical and Experimental Medicine

Article Title: TMEM132A is associated with metabolic reprogramming, macrophage-oriented immune remodeling, and breast cancer progression

doi: 10.1007/s10238-026-02125-3

Figure Lengend Snippet: TMEM132A is upregulated in breast cancer and correlates with clinicopathological features ( A ) Differential expression of TMEM132A between breast cancer tissues and normal breast tissues in the TCGA_BRCA cohort. ( B ) TMEM132A expression comparison between tumor samples from TCGA_BRCA and normal breast tissues from the integrated TCGA and GTEx cohort. ( C-E ) Validation of elevated TMEM132A expression in breast cancer using independent GEO datasets, including GSE20711 ( C ), GSE22820 ( D ), and GSE162228 ( E ). ( F , G ) ROC curve analyses evaluating the diagnostic performance of TMEM132A in distinguishing breast cancer tissues from normal tissues in the TCGA_BRCA cohort ( F ) and the TCGA+GTEx_BRCA cohort ( G ). ( H ) Paired analysis of TMEM132A expression between matched tumor and adjacent normal breast tissues in the TCGA_BRCA cohort. ( I , J ) Association between TMEM132A expression and clinical stage in the TCGA_BRCA cohort. ( K ) Distribution of TMEM132A expression across different molecular subtypes of breast cancer in the TCGA_BRCA cohort. ( L-T ) Relationship between TMEM132A expression and histological grade in the TCGA_BRCA cohort (L) and multiple independent GEO datasets, including GSE7390 ( M ), GSE11121 ( N ), GSE20711 ( O ), GSE21653 ( P ), GSE22219 ( Q ), GSE25055 ( R ), GSE45255 ( S ), GSE42568 ( T ), and GSE61304 ( U ). ( U , V ) Comparison of TMEM132A expression between non-metastatic (M0) and metastatic (M1) breast cancer samples in the TCGA_BRCA cohort ( U ) and the GSE20685 dataset ( V ). ( W ) Distribution of TMEM132A expression levels across breast cancer molecular. subgroups in the TCGA_BRCA cohort. ( X ) Representative immunohistochemical staining images showing TMEM132A protein expression in normal breast tissue and breast cancer tissue obtained from the Human Protein Atlas. ( Y ) Immunofluorescence staining showing TMEM132A expression (green), nuclei (blue), microtubules (red), and merged images in the MCF7 breast cancer cell line

Article Snippet: Fig. 2 TMEM132A is upregulated in breast cancer and correlates with clinicopathological features ( A ) Differential expression of TMEM132A between breast cancer tissues and normal breast tissues in the TCGA_BRCA cohort. ( B ) TMEM132A expression comparison between tumor samples from TCGA_BRCA and normal breast tissues from the integrated TCGA and GTEx cohort. ( C-E ) Validation of elevated TMEM132A expression in breast cancer using independent GEO datasets, including GSE20711 ( C ), GSE22820 ( D ), and GSE162228 ( E ). ( F , G ) ROC curve analyses evaluating the diagnostic performance of TMEM132A in distinguishing breast cancer tissues from normal tissues in the TCGA_BRCA cohort ( F ) and the TCGA+GTEx_BRCA cohort ( G ). ( H ) Paired analysis of TMEM132A expression between matched tumor and adjacent normal breast tissues in the TCGA_BRCA cohort. ( I , J ) Association between TMEM132A expression and clinical stage in the TCGA_BRCA cohort. ( K ) Distribution of TMEM132A expression across different molecular subtypes of breast cancer in the TCGA_BRCA cohort. ( L-T ) Relationship between TMEM132A expression and histological grade in the TCGA_BRCA cohort (L) and multiple independent GEO datasets, including GSE7390 ( M ), GSE11121 ( N ), GSE20711 ( O ), GSE21653 ( P ), GSE22219 ( Q ), GSE25055 ( R ), GSE45255 ( S ), GSE42568 ( T ), and GSE61304 ( U ). ( U , V ) Comparison of TMEM132A expression between non-metastatic (M0) and metastatic (M1) breast cancer samples in the TCGA_BRCA cohort ( U ) and the GSE20685 dataset ( V ). ( W ) Distribution of TMEM132A expression levels across breast cancer molecular. subgroups in the TCGA_BRCA cohort. ( X ) Representative immunohistochemical staining images showing TMEM132A protein expression in normal breast tissue and breast cancer tissue obtained from the Human Protein Atlas. ( Y ) Immunofluorescence staining showing TMEM132A expression (green), nuclei (blue), microtubules (red), and merged images in the MCF7 breast cancer cell line We next assessed the potential diagnostic value of TMEM132A in distinguishing tumor from normal tissues.

Techniques: Quantitative Proteomics, Expressing, Comparison, Biomarker Discovery, Diagnostic Assay, Immunohistochemical staining, Staining, Immunofluorescence